This TEEB Manual provides both technical and operational guidance on how countries may conduct a TEEB Country Study. It outlines the various steps that may be taken to initiate and implement a country study, communicate its findings, and implement the recommendations of the study. This manual is designed for anyone who is considering or currently undertaking a TEEB country study (TCS). Its purpose is to provide guidance throughout the entire TCS cycle i.e., from initiation, to policy analysis and ecosystem service valuations, communicating findings, following up and finally applying results. The manual is not intended to be prescriptive but rather provides directions and guidelines for TEEB implementation, which must be adapted according to the specific circumstances and objectives of the country. It also outlines the quality and completeness criteria for such a study to become formally endorsed as a “TEEB Country Study” by the Advisory Board of the international TEEB initiative. The Guidance Manual was developed following requests from countries interested in undertaking a TEEB country study, in order to achieve their development goals whilst at the same time sustainably managing their natural resources.